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Read e-book online Advances in Applied Biotechnology: Proceedings of the 2nd PDF

By Tong-Cun Zhang, Motowo Nakajima

ISBN-10: 3662456567

ISBN-13: 9783662456569

ISBN-10: 3662456575

ISBN-13: 9783662456576

At the ICAB 2014, researchers from world wide will assemble to debate the newest clinical learn, findings and applied sciences relating Microbial Genetics and Breeding, Optimization and keep an eye on of organic strategies, organic Separation and organic Purification, and Advances in Biotechnology.
This convention will offer a platform for educational trade at the program of biotechnology among family and overseas universities, learn institutes, company specialists and students. The contributors will specialise in the overseas improvement and destiny traits. the development will lay a high-quality beginning for addressing key technical demanding situations in numerous parts of utilized biotechnology, supplying possibilities to advertise the advance and enlargement of the biotechnology industry.

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Length (bp) 137 555 471 366 382 556 203 Min. 3 Annotation of three DEGs using BLAST2GO Seq. name Seq. description #Hits Min. 95 10 P: cell redox homeostasis; F: electron carrier activity; P: glycerol ether metabolic process; F: protein disulfide oxidoreductase activity; C: cytoplasm; P: electron transport chain; P: transport F: nutrient reservoir activity F: ATPase activity; F: ATP binding; F: hydrolase activity; F: nucleotide binding; F: nucleosidetriphosphatase activity; F: phosphonate transmembrane-transporting ATPase activity; P: ATP catabolic process; F: transporter activity; C:mitochondrion; C: plastid P1-89-1-5 5 Isolation of Differentially Expressed Genes from Developing … 43 Fig.

The SSR marker is of high cost. SRAP, which possesses advantages over other molecular markers, possesses extraordinary characters such as simple design of primers, low cost, stable amplification, and high polymorphism. It is a simple, economic, effective, and reliable molecular marker. In order to screen molecular markers of salt-tolerant genes, 225 pair primers, which were designed, were mined using genomic DNA of maize. The 48 pairs of primers could amplify stable and clear bands. Six pairs of primers could amplify polymorphic PCR products among parent N1, parent M2, salt tolerant DNA pool, and susceptible DNA pool.

No molecular marker or gene associated with this important trait of groundnut has been documented. In the present study, 105 unique cDNA fragments were isolated from two groundnut materials with contrasting dormancy phenotypes using GeneFishing technology. Three of the 16 DEGs were related to seed germination as suggested by BLAST2GO analysis. Differential expression of a gene with functions indicated in seed germination [85-3-1 (oleosin kda-like)] was confirmed by qRT-PCR. We postulate that expression of the gene is positively related to intensity of groundnut seed dormancy, and that the gene product protects oil bodies thereby preventing lipid mobilization from providing energy for germination.

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Advances in Applied Biotechnology: Proceedings of the 2nd International Conference on Applied Biotechnology (ICAB 2014)-Volume I by Tong-Cun Zhang, Motowo Nakajima

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